Circulating Nucleic Acids in Plasma Or SerumPhilippe Anker, Maurice Stroun New York Academy of Sciences, 2000 - 188 Free DNA present in both healthy and diseased human plasma has been found to express specific point mutations that may serve as diagnostic signposts - perhaps to reliable, non-invasive tests for breast and lung cancer, haematopoetic malignancies, colorectal or pancreatic carcinoma, and other tumours. In another research area, foetal DNA has been detected in maternal serum which presents an early-horizon alternative in utero test for gender. A parallel discovery of rearranged immunoglobin heavy-chain DNA in the plasma of patients wit Hodgkin's disease or acute B cell leukaemia has also emerged. These fast-emerging indicators suggest that it is time to standardize research techniques and develop protocols that can take a measure of circulating DNA's usefulness as a diagnostic tool. This volume, taken from the proceedings of a conference in August 1999, discusses such issues. |
Spis treści
Quantitative Aspects of PlasmaSerum DNA in Cancer Patients | 5 |
A Review of Studies on the Detection of Mutated KRAS2 Sequences | 13 |
Diagnostic Value of Kras Mutations in Serum of Pancreatic Cancer | 19 |
Prawa autorskie | |
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alleles amplification ANKER apoptosis bone marrow Cancer Res carcinoma cell lung cancer cell-free fetal DNA chromosome circulating DNA Clin clinical codon colorectal cancer correlation diagnosis disease DNA extracted DNA in maternal DNA levels Exon extracellular fetal cells genetic alterations genome human K-ras gene K-ras mutations LEDERREY lesions lung cancer lung cancer patients LYAUTEY lymphocytes malignant maternal plasma melanoma metastasis metastatic method Microsatellite alterations microsatellite analysis Microsatellite instability microsatellite markers molecular mRNA mutated KRAS2 mutation detection nested PCR normal Nucleic Acids oligonucleotide oncogene Oncol pancreatic cancer patients with colorectal patients with pancreatic PCR products peripheral blood plasma and serum plasma DNA plasma of patients plasma or serum plasma/serum plasmids polymerase chain reaction prenatal primary tumor primers protein RT-PCR sensitivity sepsis serum DNA serum of patients SIDRANSKY specific stage STROUN studies supernat tion tissue tumor cells tumor DNA tumor markers tumor suppressor genes urine wild-type